A C/EBP beta isoform recruits the SWI/SNF complex to activate myeloid genes.
The activation of many genes requires the concerted effort of two or more transcription factors. Although C/EBP beta is known to cooperate with Myb to induce transcription of the granulocyte-specific mim-1 gene, the molecular mechanism of this cooperativity is undefined. We show that the N terminus of the full-length C/EBP ... beta isoform, which is essential for induction of the mim-1 gene in chromatin, interacts specifically with the SWI/SNF complex. Grafting this domain onto Myb generates a chimeric activator that recruits SWI/SNF and induces mim-1 transcription in the absence of C/EBP beta. Interaction between C/EBP beta and SWI/SNF is essential for activating a subgroup of resident target genes in chromatin and may represent a major determinant of combinatorial gene regulation in eukaryotes.
Mesh Terms:
Acetyltransferases, Adenosine Triphosphatases, Animals, Binding Sites, CCAAT-Enhancer-Binding Proteins, Cell Cycle Proteins, Cell Line, Chromatin, DNA Helicases, DNA-Binding Proteins, Drosophila Proteins, Erythroblasts, Fibroblasts, Humans, Multienzyme Complexes, Nuclear Proteins, Promoter Regions, Genetic, Protein Binding, Protein Isoforms, Proteins, Proto-Oncogene Proteins c-myb, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Deletion, Trans-Activators, Transcription Factors, Transcriptional Activation
Acetyltransferases, Adenosine Triphosphatases, Animals, Binding Sites, CCAAT-Enhancer-Binding Proteins, Cell Cycle Proteins, Cell Line, Chromatin, DNA Helicases, DNA-Binding Proteins, Drosophila Proteins, Erythroblasts, Fibroblasts, Humans, Multienzyme Complexes, Nuclear Proteins, Promoter Regions, Genetic, Protein Binding, Protein Isoforms, Proteins, Proto-Oncogene Proteins c-myb, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Deletion, Trans-Activators, Transcription Factors, Transcriptional Activation
Mol. Cell
Date: Nov. 01, 1999
PubMed ID: 10619021
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