Inhibition of E2F-mediated transcription by p202.
Many of the antimicrobial, immunomodulatory and cell growth inhibitory activities of the interferons are mediated by interferon-inducible proteins. Earlier we characterized an interferon-inducible murine protein, p202, whose expression in transfected cells inhibits cell proliferation and which can form a complex with retinoblastoma protein (pRb). Here we report that in transfected ... cells expression of p202 inhibits E2F-stimulated transcription of a reporter gene and of endogenous genes. Inhibition of the transcriptional activity of E2F by p202 does not depend on fully functional pRb and is correlated with inhibition of the sequence-specific DNA binding of E2F. p202 interacts with the transcription factor E2F (E2F-1/DP-1) in vitro and in vivo. Inhibition of E2F activity by p202 may contribute to growth inhibition by the interferons.
Mesh Terms:
Animals, Carrier Proteins, Cell Cycle Proteins, Cell Division, DNA, DNA-Binding Proteins, E2F Transcription Factors, E2F1 Transcription Factor, Electrophoresis, Polyacrylamide Gel, Female, Genes, Reporter, Hela Cells, Humans, Interferons, Intracellular Signaling Peptides and Proteins, Mice, Nuclear Proteins, Phosphoproteins, Retinoblastoma-Binding Protein 1, Transcription Factor DP1, Transcription Factors, Transcription, Genetic, Tumor Cells, Cultured
Animals, Carrier Proteins, Cell Cycle Proteins, Cell Division, DNA, DNA-Binding Proteins, E2F Transcription Factors, E2F1 Transcription Factor, Electrophoresis, Polyacrylamide Gel, Female, Genes, Reporter, Hela Cells, Humans, Interferons, Intracellular Signaling Peptides and Proteins, Mice, Nuclear Proteins, Phosphoproteins, Retinoblastoma-Binding Protein 1, Transcription Factor DP1, Transcription Factors, Transcription, Genetic, Tumor Cells, Cultured
EMBO J.
Date: Oct. 15, 1996
PubMed ID: 8896460
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