Hypoxia increases the association of 4E-binding protein 1 with the initiation factor 4E in isolated rat hepatocytes.

Incubation of hepatocytes under hypoxia increases binding of translation initiation factor eIF-4E to its inhibitory regulator 4E-BP1, and this correlates with dephosphorylation of 4E-BP1. Rapamycin induced the same effect in aerobic cells but no additive effect was observed when hypoxic cells were treated with rapamycin. This enhanced association of 4E-BP1 ...
with eIF-4E might be mediated by mTOR. Nevertheless, only hypoxia produces a rapid inhibition of protein synthesis. Although hypoxia might be signalling via the rapamycin-sensitive pathway by changing eIF-4E availability, such a pathway is unlikely to be responsible for the depression in overall protein synthesis under hypoxia.
Mesh Terms:
Animals, Antifungal Agents, Carrier Proteins, Cell Hypoxia, Cells, Cultured, Eukaryotic Initiation Factor-4E, Liver, Male, Peptide Initiation Factors, Phosphoproteins, Phosphorylation, Rats, Rats, Wistar, Signal Transduction, Sirolimus
FEBS Lett.
Date: Mar. 05, 1999
Download Curated Data For This Publication
77888
Switch View:
  • Interactions 1