From androgen receptor to the general transcription factor TFIIH. Identification of cdk activating kinase (CAK) as an androgen receptor NH(2)-terminal associated coactivator.

The androgen receptor (AR), like other steroid receptors, modulates the activity of the general transcription machinery on the core promoter to exert its function as a regulator. Co-immunoprecipitation of prostate cancer LNCaP cell extract using protein A-Sepharose coupled with anti-AR antibody indicates that the AR interacts with the general transcription ...
factor TFIIH in a physiological condition. Co-transfection of cdk activating kinase (CAK), the kinase moiety of TFIIH, enhanced AR-mediated transcription in a ligand-dependent manner in human prostate cancer PC-3 and LNCaP cells, and in a ligand-independent manner in human prostate cancer DU145 cells. Detailed interaction studies further revealed that the AR NH(2)-terminal domain interacting with CAK was essential for the CAK-induced AR transactivation. Together, our data suggest that the AR may interact with TFIIH for efficient communication with the general transcription factors/RNA polymerase II on the core promoter.
Mesh Terms:
Cyclin H, Cyclin-Dependent Kinases, Cyclins, DNA-Binding Proteins, Fungal Proteins, Humans, Male, Prostatic Neoplasms, Protein-Serine-Threonine Kinases, Receptors, Androgen, Recombinant Fusion Proteins, Saccharomyces cerevisiae Proteins, TATA-Binding Protein Associated Factors, Transcription Factor TFIID, Transcription Factor TFIIH, Transcription Factors, Transcription Factors, TFII, Tumor Cells, Cultured
J. Biol. Chem.
Date: Mar. 31, 2000
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