Specific association of Type I c-Abl with Ran GTPase in lipopolysaccharide-mediated differentiation.
Each of several isoforms of c-Abl may be involved in different biological functions. Type I c-Abl has been shown to be involved in LPS-induced differentiation and Type IV c-Abl, apoptosis. Ran has recently been shown to be involved in LPS endotoxin signal transduction. Here we show that Type I c-Abl ... associates with Ran. Formation of this complex is specific, as Ran did not associate with the highly homologous Type IV c-Abl isoform. In non-stimulated lymphoid B cells, Type I c-Abl tyrosine kinase is inactive, whereas Type IV kinase is active. Formation of Type I c-Abl/Ran complex and activation of Type I c-Abl kinase activity are LPS dose-dependent. This complex is detectable in B cells of endotoxin-sensitive inbred mice but absent in B cells of endotoxin-resistant mice. These findings therefore suggest that Type I c-Abl and Ran are important targets in lipopolysaccharide-induced biological responses of hematopoietic cells.
Mesh Terms:
Animals, B-Lymphocytes, Base Sequence, Cell Differentiation, Cell Line, Enzyme Activation, GTP Phosphohydrolases, Lipopolysaccharides, Lymphocyte Activation, Mice, Mice, Inbred C3H, Oligonucleotides, Antisense, Precipitin Tests, Protein Isoforms, Protein-Tyrosine Kinases, Proto-Oncogene Proteins c-abl, Signal Transduction, ran GTP-Binding Protein
Animals, B-Lymphocytes, Base Sequence, Cell Differentiation, Cell Line, Enzyme Activation, GTP Phosphohydrolases, Lipopolysaccharides, Lymphocyte Activation, Mice, Mice, Inbred C3H, Oligonucleotides, Antisense, Precipitin Tests, Protein Isoforms, Protein-Tyrosine Kinases, Proto-Oncogene Proteins c-abl, Signal Transduction, ran GTP-Binding Protein
Oncogene
Date: May. 10, 2001
PubMed ID: 11420673
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