NADP regulates the yeast GAL induction system.
Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UAS(GAL)); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and ... adenosine 5'-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial induction event.
Mesh Terms:
Amino Acid Motifs, Binding Sites, Crystallography, X-Ray, DNA-Binding Proteins, Dimerization, Galactokinase, Galactose, Gene Expression Regulation, Fungal, Models, Molecular, NADP, Protein Structure, Secondary, Protein Structure, Tertiary, Recombinant Fusion Proteins, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcription, Genetic
Amino Acid Motifs, Binding Sites, Crystallography, X-Ray, DNA-Binding Proteins, Dimerization, Galactokinase, Galactose, Gene Expression Regulation, Fungal, Models, Molecular, NADP, Protein Structure, Secondary, Protein Structure, Tertiary, Recombinant Fusion Proteins, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcription, Genetic
Science
Date: Feb. 22, 2008
PubMed ID: 18292341
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