MM-1, a novel c-Myc-associating protein that represses transcriptional activity of c-Myc.
We have isolated the cDNA encoding a novel c-Myc-binding protein, MM-1, by the yeast two-hybrid screening of a human HeLa cell cDNA library. The protein deduced from the cDNA comprises 167 amino acids and was localized in the nucleus of introduced COS-I cells. The MM-1 mRNA was highly expressed in ... human pancreas and skeletal muscle and moderately in other tissues. As for the c-Myc binding, glutathione S-transferase MM-1 expressed in Escherichia coli bound in vitro to c-Myc translated in reticulocyte lysate, and almost whole, the MM-1 molecule was necessary for the binding in the yeast two-hybrid system. The mammalian two-hybrid assays in hamster CHO cells revealed that MM-1 interacts in vivo with the N-terminal domain covering the myc box 2, a transcription-activating domain, of c-Myc. Furthermore, MM-1 repressed the activation of E-box-dependent transcription by c-Myc.
Mesh Terms:
Amino Acid Sequence, Animals, Base Sequence, CHO Cells, COS Cells, Cloning, Molecular, Cricetinae, DNA Primers, DNA, Complementary, Fluorescent Antibody Technique, Indirect, Hela Cells, Humans, Molecular Sequence Data, Proto-Oncogene Proteins c-myc, Repressor Proteins, Saccharomyces cerevisiae, Transcription, Genetic
Amino Acid Sequence, Animals, Base Sequence, CHO Cells, COS Cells, Cloning, Molecular, Cricetinae, DNA Primers, DNA, Complementary, Fluorescent Antibody Technique, Indirect, Hela Cells, Humans, Molecular Sequence Data, Proto-Oncogene Proteins c-myc, Repressor Proteins, Saccharomyces cerevisiae, Transcription, Genetic
J. Biol. Chem.
Date: Nov. 06, 1998
PubMed ID: 9792694
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