Association of Src tyrosine kinase with a human potassium channel mediated by SH3 domain.
The human Kv1.5 potassium channel (hKv1.5) contains proline-rich sequences identical to those that bind to Src homology 3 (SH3) domains. Direct association of the Src tyrosine kinase with cloned hKv1.5 and native hKv1.5 in human myocardium was observed. This interaction was mediated by the proline-rich motif of hKv1.5 and the ... SH3 domain of Src. Furthermore, hKv1.5 was tyrosine phosphorylated, and the channel current was suppressed, in cells coexpressing v-Src. These results provide direct biochemical evidence for a signaling complex composed of a potassium channel and a protein tyrosine kinase.
Mesh Terms:
Amino Acid Sequence, Cell Line, Cloning, Molecular, Humans, Kv1.5 Potassium Channel, Molecular Sequence Data, Myocardium, Oncogene Protein pp60(v-src), Patch-Clamp Techniques, Phosphorylation, Phosphotyrosine, Potassium Channels, Potassium Channels, Voltage-Gated, Recombinant Fusion Proteins, Signal Transduction, Transfection, src Homology Domains, src-Family Kinases
Amino Acid Sequence, Cell Line, Cloning, Molecular, Humans, Kv1.5 Potassium Channel, Molecular Sequence Data, Myocardium, Oncogene Protein pp60(v-src), Patch-Clamp Techniques, Phosphorylation, Phosphotyrosine, Potassium Channels, Potassium Channels, Voltage-Gated, Recombinant Fusion Proteins, Signal Transduction, Transfection, src Homology Domains, src-Family Kinases
Science
Date: Dec. 20, 1996
PubMed ID: 8953041
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