Structure-system correlation identifies a gene regulatory Mediator submodule.
A combination of crystallography, biochemistry, and gene expression analysis identifies the coactivator subcomplex Med8C/18/20 as a functionally distinct submodule of the Mediator head module. Med8C forms a conserved alpha-helix that tethers Med18/20 to the Mediator. Deletion of Med8C in vivo results in dissociation of Med18/20 from Mediator and in loss ... of transcription activity of extracts. Deletion of med8C, med18, or med20 causes similar changes in the yeast transcriptome, establishing Med8C/18/20 as a predominantly positive, gene-specific submodule required for low transcription levels of nonactivated genes, including conjugation genes. The presented structure-based system perturbation is superior to gene deletion analysis of gene regulation.
Mesh Terms:
Electrophoresis, Polyacrylamide Gel, Gene Deletion, Gene Expression Profiling, Gene Expression Regulation, Fungal, Mass Spectrometry, Mediator Complex, Models, Biological, Models, Molecular, Protein Structure, Tertiary, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Structure-Activity Relationship, Transcription Factors, Transcription, Genetic
Electrophoresis, Polyacrylamide Gel, Gene Deletion, Gene Expression Profiling, Gene Expression Regulation, Fungal, Mass Spectrometry, Mediator Complex, Models, Biological, Models, Molecular, Protein Structure, Tertiary, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Structure-Activity Relationship, Transcription Factors, Transcription, Genetic
Genes Dev.
Date: Apr. 01, 2008
PubMed ID: 18381891
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