Beta-arrestin 2: a receptor-regulated MAPK scaffold for the activation of JNK3.

beta-Arrestins, originally discovered in the context of heterotrimeric guanine nucleotide binding protein-coupled receptor (GPCR) desensitization, also function in internalization and signaling of these receptors. We identified c-Jun amino-terminal kinase 3 (JNK3) as a binding partner of beta-arrestin 2 using a yeast two-hybrid screen and by coimmunoprecipitation from mouse brain extracts ...
or cotransfected COS-7 cells. The upstream JNK activators apoptosis signal-regulating kinase 1 (ASK1) and mitogen-activated protein kinase (MAPK) kinase 4 were also found in complex with beta-arrestin 2. Cellular transfection of beta-arrestin 2 caused cytosolic retention of JNK3 and enhanced JNK3 phosphorylation stimulated by ASK1. Moreover, stimulation of the angiotensin II type 1A receptor activated JNK3 and triggered the colocalization of beta-arrestin 2 and active JNK3 to intracellular vesicles. Thus, beta-arrestin 2 acts as a scaffold protein, which brings the spatial distribution and activity of this MAPK module under the control of a GPCR.
Mesh Terms:
Angiotensin II, Animals, Arrestins, COS Cells, Cell Line, Cell Nucleus, Cytosol, Endosomes, Enzyme Activation, Humans, MAP Kinase Kinase 4, MAP Kinase Kinase Kinase 5, MAP Kinase Kinase Kinases, MAP Kinase Signaling System, Mice, Mitogen-Activated Protein Kinase 10, Mitogen-Activated Protein Kinase Kinases, Mitogen-Activated Protein Kinases, Mutation, Phosphorylation, Protein-Tyrosine Kinases, Proto-Oncogene Proteins c-jun, Rats, Receptor, Angiotensin, Type 1, Receptors, Angiotensin, Recombinant Fusion Proteins, Transfection, Two-Hybrid System Techniques
Science
Date: Nov. 24, 2000
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