Nuclear localization of Rad52 is pre-requisite for its sumoylation.
In Saccharomyces cerevisiae, Rad52 plays major roles in several types of homologous recombination. Here, we found that rad52-K200R mutation greatly reduced sumoylation of Rad52. The rad52-K200R mutant exhibited defects in various types of recombination, such as intrachromosomal recombination and mating-type switching. The K200 residue of Rad52 is part of the ... nuclear localization signal (NLS), which is important for transport into the nucleus. Indeed, the addition of a SV40 NLS to Rad52-K200R suppressed the sumoylation defect of Rad52-K200R. These findings indicate that nuclear localization of Rad52 is pre-requisite for its sumoylation.
Mesh Terms:
Active Transport, Cell Nucleus, Amino Acid Substitution, Arginine, Cell Nucleus, Chromatids, Deoxyribonucleases, Type II Site-Specific, Lysine, Mutation, Nuclear Localization Signals, Protein Processing, Post-Translational, Rad52 DNA Repair and Recombination Protein, Recombination, Genetic, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Active Transport, Cell Nucleus, Amino Acid Substitution, Arginine, Cell Nucleus, Chromatids, Deoxyribonucleases, Type II Site-Specific, Lysine, Mutation, Nuclear Localization Signals, Protein Processing, Post-Translational, Rad52 DNA Repair and Recombination Protein, Recombination, Genetic, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Biochem. Biophys. Res. Commun.
Date: Jul. 18, 2008
PubMed ID: 18482582
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