The fission yeast homologue of Glel is essential for growth and involved in mRNA export.
We have isolated Glel homologue (named as spglel) as a partial multicopy suppressor of the synthetic lethality of rael-167 elfl-21 in fission yeast Schizosaccharomyces pombe. The spglel is also able to complement partially temperature-sensitive phenotype of rael-167 only at a lower restrictive temperature. The spglel gene contains one intron and ... encodes a 480 amino-acid protein with predicted molecular weight of 56.2 kDa. We showed that spglel gene is essential for vegetative growth and functional Glel-GFP protein is localized mainly in NPC. The accumulation of poly(A)(+) RNA in the nucleus is exhibited when expression of spglel is repressed or over-expressed. These results suggest that the spGle1 protein is also involved in mRNA export in fission yeast.
Mesh Terms:
Cell Nucleus, Gene Expression, Genes, Suppressor, Nuclear Proteins, RNA Transport, RNA, Fungal, RNA, Messenger, Schizosaccharomyces, Schizosaccharomyces pombe Proteins
Cell Nucleus, Gene Expression, Genes, Suppressor, Nuclear Proteins, RNA Transport, RNA, Fungal, RNA, Messenger, Schizosaccharomyces, Schizosaccharomyces pombe Proteins
J. Microbiol.
Date: Aug. 01, 2008
PubMed ID: 18758733
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