BPOZ-2 directly binds to eEF1A1 to promote eEF1A1 ubiquitylation and degradation and prevent translation.
Bood POZ containing gene type 2 (BPOZ-2), which contains ankyrin repeats, NLS, BTB/POZ domains and LXXLL motifs, is an adaptor protein for the E3 ubiquitin ligase scaffold protein CUL3. We isolated a cDNA encoding eukaryotic elongation factor 1A1 (eEF1A1) as a BPOZ-2 binding protein by screening a human thymus cDNA ... library using a yeast two-hybrid system. eEF1A1 is essential for translation and is also involved in the 26S proteasome-dependent degradation of misfolded or unfolded proteins. The binding between BPOZ-2 and eEF1A1 was confirmed by pull-down and immunoprecipitation assays in vitro and in vivo, respectively. BPOZ-2 binds to eEF1A1 through the ankyrin repeats and both BTB/POZ domains in BPOZ-2 and Domains I and III in eEF1A1. BPOZ-2 and eEF1A1 over-expressed in HEK 293T cells co-localized as speckles within the cytoplasm. BPOZ-2 promoted eEF1A1 ubiquitylation and degradation, suggesting that eEF1A1 is a substrate of BPOZ-2. BPOZ-2 inhibited GTP binding to eEF1A1 and prevented translation in in vitro translation assay using rabbit reticulocytes.
Mesh Terms:
Animals, COS Cells, Cattle, Cell Line, Cell-Free System, Cercopithecus aethiops, Humans, Mutagenesis, Site-Directed, Peptide Elongation Factor 1, Proteasome Endopeptidase Complex, Protein Biosynthesis, Repressor Proteins, Thymus Gland, Transfection, Two-Hybrid System Techniques, Ubiquitination
Animals, COS Cells, Cattle, Cell Line, Cell-Free System, Cercopithecus aethiops, Humans, Mutagenesis, Site-Directed, Peptide Elongation Factor 1, Proteasome Endopeptidase Complex, Protein Biosynthesis, Repressor Proteins, Thymus Gland, Transfection, Two-Hybrid System Techniques, Ubiquitination
Genes Cells
Date: Jun. 01, 2008
PubMed ID: 18459963
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- Interactions 3
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