Activation of the ERK signal transduction pathway by Epstein-Barr virus immediate-early protein Rta.
BRCA1-associated protein 2 (BRAP2) is known to interact with the kinase suppressor of Ras 1 (KSR1), inhibiting the ERK signal transduction cascade. This study found that an Epstein-Barr virus (EBV) immediate-early protein, Rta, is a binding partner of BRAP2 in yeast and confirmed the binding in vitro by a glutathione ... S-transferase pull-down assay and in vivo by co-immunoprecipitation in 293(maxi-EBV) cells. Binding studies also showed that Rta and KSR1 interacted with the C-terminal 202 aa region in BRAP2. Additionally, Rta appeared to prevent the binding of KSR1 to BRAP2, activating the ERK signal transduction pathway and the transcription of an EBV immediate-early gene, BZLF1. Activation of the ERK signal transduction pathway by Rta may be critical for the maintenance of the lytic state of EBV.
Mesh Terms:
Binding, Competitive, Cell Line, Extracellular Signal-Regulated MAP Kinases, Gene Expression Regulation, Viral, Herpesvirus 4, Human, Humans, Immediate-Early Proteins, Promoter Regions, Genetic, Protein Binding, Protein Kinases, Signal Transduction, Trans-Activators, Transcription, Genetic, Transcriptional Activation, Two-Hybrid System Techniques, Ubiquitin-Protein Ligases
Binding, Competitive, Cell Line, Extracellular Signal-Regulated MAP Kinases, Gene Expression Regulation, Viral, Herpesvirus 4, Human, Humans, Immediate-Early Proteins, Promoter Regions, Genetic, Protein Binding, Protein Kinases, Signal Transduction, Trans-Activators, Transcription, Genetic, Transcriptional Activation, Two-Hybrid System Techniques, Ubiquitin-Protein Ligases
J. Gen. Virol.
Date: Oct. 01, 2008
PubMed ID: 18796711
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