Identification of Tyr-703 and Tyr-936 as the primary association sites for Grb2 and Grb7 in the c-Kit/stem cell factor receptor.
In this paper we demonstrate the presence of two novel in vivo autophosphorylation sites in the c-Kit/stem cell factor receptor (c-Kit/SCFR): Tyr-703 in the kinase insert and Tyr-936 in the C-terminal tail. We furthermore demonstrate that the adapter protein Grb2 is a specific binding partner for both phosphorylated Tyr-703 and ... phosphorylated Tyr-936, whereas the adapter protein Grb7 binds selectively to phosphorylated Tyr-936. It is shown that the association occurs through the Src homology 2 (SH2) domains of Grb2 and Grb7. Binding of Grb2 to Tyr-703 in the c-Kit/SCFR provides a link to the Ras/mitogen-activated protein kinase pathway.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Cells, Cultured, Endothelium, Vascular, GRB2 Adaptor Protein, GRB7 Adaptor Protein, Humans, Phosphorylation, Protein Binding, Proteins, Proto-Oncogene Proteins c-kit, Receptor Protein-Tyrosine Kinases, Recombinant Fusion Proteins, Stem Cell Factor, Tyrosine, src Homology Domains
Adaptor Proteins, Signal Transducing, Cells, Cultured, Endothelium, Vascular, GRB2 Adaptor Protein, GRB7 Adaptor Protein, Humans, Phosphorylation, Protein Binding, Proteins, Proto-Oncogene Proteins c-kit, Receptor Protein-Tyrosine Kinases, Recombinant Fusion Proteins, Stem Cell Factor, Tyrosine, src Homology Domains
Biochem. J.
Date: Jul. 01, 1999
PubMed ID: 10377264
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