hADA3 is required for p53 activity.
The tumor suppressor protein p53 is a transcription factor that is frequently mutated in human cancers. In response to DNA damage, p53 protein is stabilized and activated by post-translational modifications that enable it to induce either apoptosis or cell cycle arrest. Using a novel yeast p53 dissociator assay, we identify ... hADA3, a part of histone acetyltransferase complexes, as an important cofactor for p53 activity. p53 and hADA3 physically interact in human cells. This interaction is enhanced dramatically after DNA damage due to phosphorylation event(s) in the p53 N-terminus. Proper hADA3 function is essential for full transcriptional activity of p53 and p53-mediated apoptosis.
Mesh Terms:
Acetyltransferases, Antibiotics, Antineoplastic, Apoptosis, Cell Line, Cells, Cultured, DNA, DNA Damage, DNA, Complementary, Dose-Response Relationship, Drug, Doxorubicin, Flow Cytometry, Gene Library, Genes, Reporter, Genes, p53, Hela Cells, Histone Acetyltransferases, Humans, Models, Biological, Mutation, Oligonucleotides, Antisense, Phosphorylation, Plasmids, Precipitin Tests, Protein Binding, Protein Processing, Post-Translational, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcription, Genetic, Transfection, Tumor Cells, Cultured, Tumor Suppressor Protein p53, Ultraviolet Rays
Acetyltransferases, Antibiotics, Antineoplastic, Apoptosis, Cell Line, Cells, Cultured, DNA, DNA Damage, DNA, Complementary, Dose-Response Relationship, Drug, Doxorubicin, Flow Cytometry, Gene Library, Genes, Reporter, Genes, p53, Hela Cells, Histone Acetyltransferases, Humans, Models, Biological, Mutation, Oligonucleotides, Antisense, Phosphorylation, Plasmids, Precipitin Tests, Protein Binding, Protein Processing, Post-Translational, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcription, Genetic, Transfection, Tumor Cells, Cultured, Tumor Suppressor Protein p53, Ultraviolet Rays
EMBO J.
Date: Nov. 15, 2001
PubMed ID: 11707411
View in: Pubmed Google Scholar
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