MafG controls the hypoxic response of cells by accumulating HIF-1alpha in the nuclei.
We identified MafG as a protein that interacts with HIF-1alpha, a key factor in hypoxic response, using the yeast two-hybrid system. Interaction between MafG and HIF-1alpha was confirmed by surface plasmon resonance and by translocation to the nucleolus with the NoLS signal. A knockdown of MafG reduced erythropoietin (EPO) mRNA ... levels as well as luciferase reporter activity with the hypoxia response element. The knockdown of MafG did not change total HIF-1alpha protein, but reduced the accumulation of HIF-1alpha in the nuclei. These results suggest that MafG regulates the hypoxic response of cells by detaining HIF-1alpha in the nuclei.
Mesh Terms:
Cell Hypoxia, Cell Line, Tumor, Cell Nucleus, Genes, Reporter, Humans, Hypoxia-Inducible Factor 1, alpha Subunit, MafG Transcription Factor, RNA Interference, Repressor Proteins, Two-Hybrid System Techniques
Cell Hypoxia, Cell Line, Tumor, Cell Nucleus, Genes, Reporter, Humans, Hypoxia-Inducible Factor 1, alpha Subunit, MafG Transcription Factor, RNA Interference, Repressor Proteins, Two-Hybrid System Techniques
FEBS Lett.
Date: Jul. 09, 2008
PubMed ID: 18538669
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