Wild-type p53 binds to the TATA-binding protein and represses transcription.
p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does ... not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.
Mesh Terms:
Animals, Cell Nucleus, Cells, Cultured, DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Genes, Tumor Suppressor, Humans, Protein Binding, Recombinant Proteins, Species Specificity, Structure-Activity Relationship, TATA Box, TATA-Box Binding Protein, Transcription Factors, Transcription, Genetic, Tumor Suppressor Protein p53
Animals, Cell Nucleus, Cells, Cultured, DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Genes, Tumor Suppressor, Humans, Protein Binding, Recombinant Proteins, Species Specificity, Structure-Activity Relationship, TATA Box, TATA-Box Binding Protein, Transcription Factors, Transcription, Genetic, Tumor Suppressor Protein p53
Proc. Natl. Acad. Sci. U.S.A.
Date: Dec. 15, 1992
PubMed ID: 1465435
View in: Pubmed Google Scholar
Download Curated Data For This Publication
8132
Switch View:
- Interactions 1