Neuropilin-1 is expressed by endothelial and tumor cells as an isoform-specific receptor for vascular endothelial growth factor.
Vascular endothelial growth factor (VEGF), a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. We now describe the purification and the expression cloning from tumor cells of a third VEGF receptor, one that binds VEGF165 but not VEGF121. This isoform-specific VEGF receptor (VEGF165R) is identical ... to human neuropilin-1, a receptor for the collapsin/semaphorin family that mediates neuronal cell guidance. When coexpressed in cells with KDR, neuropilin-1 enhances the binding of VEGF165 to KDR and VEGF165-mediated chemotaxis. Conversely, inhibition of VEGF165 binding to neuropilin-1 inhibits its binding to KDR and its mitogenic activity for endothelial cells. We propose that neuropilin-1 is a novel VEGF receptor that modulates VEGF binding to KDR and subsequent bioactivity and therefore may regulate VEGF-induced angiogenesis.
Mesh Terms:
Antigens, Surface, Cell Line, Chemotaxis, Cloning, Molecular, Endothelial Growth Factors, Endothelium, Vascular, Exons, Gene Expression, Humans, Isomerism, Lymphokines, Molecular Sequence Data, Neovascularization, Physiologic, Nerve Tissue Proteins, Neuropilin-1, RNA, Messenger, Receptor Protein-Tyrosine Kinases, Receptors, Growth Factor, Receptors, Mitogen, Receptors, Vascular Endothelial Growth Factor, Recombinant Fusion Proteins, Umbilical Cord, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factors
Antigens, Surface, Cell Line, Chemotaxis, Cloning, Molecular, Endothelial Growth Factors, Endothelium, Vascular, Exons, Gene Expression, Humans, Isomerism, Lymphokines, Molecular Sequence Data, Neovascularization, Physiologic, Nerve Tissue Proteins, Neuropilin-1, RNA, Messenger, Receptor Protein-Tyrosine Kinases, Receptors, Growth Factor, Receptors, Mitogen, Receptors, Vascular Endothelial Growth Factor, Recombinant Fusion Proteins, Umbilical Cord, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factors
Cell
Date: Mar. 20, 1998
PubMed ID: 9529250
View in: Pubmed Google Scholar
Download Curated Data For This Publication
8236
Switch View:
- Interactions 2