Reelin is a ligand for lipoprotein receptors.
A signaling pathway involving the extracellular protein Reelin and the intracellular adaptor protein Disabled-1 (Dab1) controls cell positioning during mammalian brain development. Here, we demonstrate that Reelin binds directly to lipoprotein receptors, preferably the very low-density lipoprotein receptor (VLDLR) and apolipoprotein E receptor 2 (ApoER2). Binding requires calcium, and it ... is inhibited in the presence of apoE. Furthermore, the CR-50 monoclonal antibody, which inhibits Reelin function, blocks the association of Reelin with VLDLR. After binding to VLDLR on the cell surface, Reelin is internalized into vesicles. In dissociated neurons, apoE reduces the level of Reelin-induced tyrosine phosphorylation of Dab1. These data suggest that Reelin directs neuronal migration by binding to VLDLR and ApoER2.
Mesh Terms:
Animals, COS Cells, Calcium, Cell Adhesion Molecules, Neuronal, Cell Line, Cells, Extracellular Matrix Proteins, Humans, LDL-Receptor Related Protein 1, Ligands, Mice, Nerve Tissue Proteins, Receptors, LDL, Receptors, Lipoprotein, Serine Endopeptidases
Animals, COS Cells, Calcium, Cell Adhesion Molecules, Neuronal, Cell Line, Cells, Extracellular Matrix Proteins, Humans, LDL-Receptor Related Protein 1, Ligands, Mice, Nerve Tissue Proteins, Receptors, LDL, Receptors, Lipoprotein, Serine Endopeptidases
Neuron
Date: Oct. 01, 1999
PubMed ID: 10571240
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