A novel UDP-glucose transferase is part of the callose synthase complex and interacts with phragmoplastin at the forming cell plate.

Using phragmoplastin as a bait, we isolated an Arabidopsis cDNA encoding a novel UDP-glucose transferase (UGT1). This interaction was confirmed by an in vitro protein--protein interaction assay using purified UGT1 and radiolabeled phragmoplastin. Protein gel blot results revealed that UGT1 is associated with the membrane fraction and copurified with the ...
product-entrapped callose synthase complex. These data suggest that UGT1 may act as a subunit of callose synthase that uses UDP-glucose to synthesize callose, a 1,3-beta-glucan. UGT1 also interacted with Rop1, a Rho-like protein, and this interaction occurred only in its GTP-bound configuration, suggesting that the plant callose synthase may be regulated by Rop1 through the interaction with UGT1. The green fluorescent protein--UGT1 fusion protein was located on the forming cell plate during cytokinesis. We propose that UGT1 may transfer UDP-glucose from sucrose synthase to the callose synthase and thus help form a substrate channel for the synthesis of callose at the forming cell plate.
Mesh Terms:
Arabidopsis, Cell Division, Cell Fractionation, Cell Wall, DNA, Complementary, Glucosyltransferases, Green Fluorescent Proteins, Luminescent Proteins, Membrane Proteins, Plant Proteins, Protein Binding, Recombinant Fusion Proteins, Schizosaccharomyces pombe Proteins, Sequence Alignment, Sequence Analysis, Protein, Two-Hybrid System Techniques
Plant Cell
Date: Apr. 01, 2001
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