Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome.
SCF ubiquitin ligases control various processes by marking regulatory proteins for ubiquitin-dependent proteolysis. To illuminate how SCF complexes are regulated, we sought proteins that interact with the human SCF component CUL1. The COP9 signalosome (CSN), a suppressor of plant photomorphogenesis, associated with multiple cullins and promoted cleavage of the ubiquitin-like ... protein NEDD8 from Schizosaccharomyces pombe CUL1 in vivo and in vitro. Multiple NEDD8-modified proteins uniquely accumulated in CSN-deficient S. pombe cells. We propose that the broad spectrum of activities previously attributed to CSN subunits--including repression of photomorphogenesis, activation of JUN, and activation of p27 nuclear export--underscores the importance of dynamic cycles of NEDD8 attachment and removal in biological regulation.
Mesh Terms:
3T3 Cells, Animals, Blotting, Western, Cell Cycle Proteins, Cullin Proteins, Fungal Proteins, Hela Cells, Humans, Mass Spectrometry, Mice, Multiprotein Complexes, Mutation, Peptide Hydrolases, Peptide Synthases, Protein Binding, Protein Processing, Post-Translational, Protein Subunits, Proteins, Recombinant Fusion Proteins, SKP Cullin F-Box Protein Ligases, Schizosaccharomyces, Substrate Specificity, Swine, Transfection, Two-Hybrid System Techniques, Ubiquitins
3T3 Cells, Animals, Blotting, Western, Cell Cycle Proteins, Cullin Proteins, Fungal Proteins, Hela Cells, Humans, Mass Spectrometry, Mice, Multiprotein Complexes, Mutation, Peptide Hydrolases, Peptide Synthases, Protein Binding, Protein Processing, Post-Translational, Protein Subunits, Proteins, Recombinant Fusion Proteins, SKP Cullin F-Box Protein Ligases, Schizosaccharomyces, Substrate Specificity, Swine, Transfection, Two-Hybrid System Techniques, Ubiquitins
Science
Date: May. 18, 2001
PubMed ID: 11337588
View in: Pubmed Google Scholar
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