PDCD2 is a negative regulator of HCF-1 (C1).
Temperature sensitive mutations in host cell factor 1 (HCF-1) arrest cells in the middle of the G1 phase of the cycle. We have shown that the highly conserved C-terminal WYF domain of HCF-1 protein interacts with the MYND domain of the PDCD2 protein. This inter-action is conserved between human HCF-1 ... and HCF-2 and the C. elegans HCF. Overexpression of PDCD2, which interacts with the N-CoR/mSin3A corepressor complexes, suppresses cotransfected HCF-1 complement-ation of a temperature lesion in the endogenous HCF-1 protein. Overexpression of domains of either PDCD2 or HCF-1, which should interfere with interactions between these two proteins, enhances the complementation.
Mesh Terms:
Amino Acid Sequence, Animals, Apoptosis Regulatory Proteins, Binding Sites, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Cells, Cultured, Gene Deletion, Genetic Complementation Test, Host Cell Factor C1, Humans, Molecular Sequence Data, Nuclear Proteins, Nuclear Receptor Co-Repressor 1, Precipitin Tests, Protein Structure, Tertiary, Proteins, Repressor Proteins, Saccharomyces cerevisiae, Sequence Homology, Amino Acid, Transcription Factors, Two-Hybrid System Techniques
Amino Acid Sequence, Animals, Apoptosis Regulatory Proteins, Binding Sites, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Cells, Cultured, Gene Deletion, Genetic Complementation Test, Host Cell Factor C1, Humans, Molecular Sequence Data, Nuclear Proteins, Nuclear Receptor Co-Repressor 1, Precipitin Tests, Protein Structure, Tertiary, Proteins, Repressor Proteins, Saccharomyces cerevisiae, Sequence Homology, Amino Acid, Transcription Factors, Two-Hybrid System Techniques
Oncogene
Date: Aug. 08, 2002
PubMed ID: 12149646
View in: Pubmed Google Scholar
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