Origin recognition complex (ORC) mediates histone 3 lysine 4 methylation through cooperation with Spp1 in Saccharomyces cerevisiae.
The heterohexameric origin recognition complex (ORC) has been implicated in many cellular activities, including DNA replication, transcriptional control, heterochromatin assembly, centromere and telomere function, and so on. Here, we report a new function for ORC in mediating histone methylation. Using the yeast two-hybrid system, we identify a physical interaction between ... Orc2p and Spp1p, a member of the Set1 complex, and we demonstrate the interaction between the endogenous ORC and Spp1p by co-immunoprecipitation from yeast extracts. Furthermore, we find that Orc2p physically interacts with trimethylated histone 3 lysine 4 (H3K4) on chromatin by co-immunoprecipitation. Finally, we show that the trimethylation of H3K4 is decreased in orc2-1 cells and abolished in orc2-1, spp1Delta double mutants. Our data reveal a novel facet of ORC in mediating histone methylation in collaboration with Spp1p and demonstrate a connection between ORC and chromatin structure via the Set1 complex.
Mesh Terms:
Chromatin, DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Fungal, Histone-Lysine N-Methyltransferase, Histones, Immunoprecipitation, Lysine, Methylation, Models, Biological, Origin Recognition Complex, Plasmids, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Temperature, Transcription Factors, Two-Hybrid System Techniques
Chromatin, DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Fungal, Histone-Lysine N-Methyltransferase, Histones, Immunoprecipitation, Lysine, Methylation, Models, Biological, Origin Recognition Complex, Plasmids, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Temperature, Transcription Factors, Two-Hybrid System Techniques
J. Biol. Chem.
Date: Dec. 05, 2008
PubMed ID: 18845545
View in: Pubmed Google Scholar
Download Curated Data For This Publication
84884
Switch View:
- Interactions 13