Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesion.
Replicated chromosomes are held together by the chromosomal cohesin complex from the time of their synthesis in S phase onward. This requires the replication fork-associated acetyl transferase Eco1, but Eco1's mechanism of action is not known. We identified spontaneous suppressors of the thermosensitive eco1-1 allele in budding yeast. An acetylation-mimicking ... mutation of a conserved lysine in cohesin's Smc3 subunit makes Eco1 dispensable for cell growth, and we show that Smc3 is acetylated in an Eco1-dependent manner during DNA replication to promote sister chromatid cohesion. A second set of eco1-1 suppressors inactivate the budding yeast ortholog of the cohesin destabilizer Wapl. Our results indicate that Eco1 modifies cohesin to stabilize sister chromatid cohesion in parallel with a cohesion establishment reaction that is in principle Eco1-independent.
Mesh Terms:
Acetylation, Acetyltransferases, Alleles, Cell Cycle Proteins, Chromatids, Chromosomal Proteins, Non-Histone, Chromosomes, Fungal, DNA Repair, DNA Replication, DNA, Fungal, Mutation, Nuclear Proteins, Protein Subunits, Proteochondroitin Sulfates, S Phase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Suppression, Genetic
Acetylation, Acetyltransferases, Alleles, Cell Cycle Proteins, Chromatids, Chromosomal Proteins, Non-Histone, Chromosomes, Fungal, DNA Repair, DNA Replication, DNA, Fungal, Mutation, Nuclear Proteins, Protein Subunits, Proteochondroitin Sulfates, S Phase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Suppression, Genetic
Science
Date: Jul. 25, 2008
PubMed ID: 18653893
View in: Pubmed Google Scholar
Download Curated Data For This Publication
85855
Switch View:
- Interactions 2