Acetylation of Smc3 by Eco1 is required for S phase sister chromatid cohesion in both human and yeast.
Sister chromatid cohesion is normally established in S phase in a process that depends on the cohesion establishment factor Eco1, a conserved acetyltransferase. However, due to the lack of known in vivo substrates, how Eco1 regulates cohesion is not understood. Here we report that yeast Eco1 and its human ortholog, ... ESCO1, both acetylate Smc3, a component of the cohesin complex that physically holds the sister chromatid together, at two conserved lysine residues. Mutating these lysine residues to a nonacetylatable form leads to increased loss of sister chromatid cohesion and genome instability in both yeast and human. In addition, we clarified that the acetyltransferase activity of Eco1 is essential for its function. Our study thus identified a molecular target for the acetyltransferase Eco1 and revealed that Smc3 acetylation is a conserved mechanism in regulating sister chromatid cohesion.
Mesh Terms:
Acetylation, Acetyltransferases, Amino Acid Sequence, Cell Cycle Proteins, Cell Line, Cell Proliferation, Cell Survival, Chromosomal Proteins, Non-Histone, Genomic Instability, Humans, Lysine, Molecular Sequence Data, Nuclear Proteins, Proteochondroitin Sulfates, S Phase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sister Chromatid Exchange, Substrate Specificity
Acetylation, Acetyltransferases, Amino Acid Sequence, Cell Cycle Proteins, Cell Line, Cell Proliferation, Cell Survival, Chromosomal Proteins, Non-Histone, Genomic Instability, Humans, Lysine, Molecular Sequence Data, Nuclear Proteins, Proteochondroitin Sulfates, S Phase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sister Chromatid Exchange, Substrate Specificity
Mol. Cell
Date: Jul. 11, 2008
PubMed ID: 18614053
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