Transient membrane recruitment of IRAK-1 in response to LPS and IL-1beta requires TNF R1.
The transcription factor NF-kappaB is an essential regulator of the innate immune response that functions as the first line of defense against infections. Activation of the innate immune response by bacterial lipopolysaccharide (LPS) triggers production of tumor necrosis factor-alpha (TNF-alpha) followed by interleukin-1 (IL-1). The IL-1 receptor associated kinase-1 (IRAK-1) ... is an integral component of the LPS, TNF-alpha, and IL-1 signaling pathways that regulate NF-kappaB. Thus we hypothesized that IRAK-1 coordinates cellular NF-kappaB responses to LPS, TNF-alpha, and IL-1. In contrast to TNF-alpha where IRAK-1 subcellular localization does not change, treatment with LPS or IL-1 leads to a loss in cytoplasmic IRAK-1 with a coordinate increase in plasma membrane associated modified IRAK-1. In fibroblasts lacking the type 1 TNF-alpha receptor (TNF R1), IRAK-1 turnover is altered and modification of IRAK-1 in the plasma membrane is decreased in response to LPS and IL-1, respectively. When NF-kappaB controlled gene expression is measured, fibroblasts lacking TNF R1 are hyperresponsive to LPS, whereas a more variable response to IL-1 is seen. Further analysis of the LPS response revealed that plasma membrane-associated IRAK-1 is found in Toll 4, IL-1, and TNF R1-containing complexes. The data presented herein suggest a model whereby the TNF R1-IRAK-1 interaction integrates the cellular response to LPS, TNF-alpha, and IL-1, culminating in a cell poised to activate TNF-alpha-dependent NF-kappaB controlled gene expression. In the absence of TNF R1-dependent events, exposure to LPS or IL-1 leads to hyperactivation of the inflammatory response.
Mesh Terms:
Animals, Cell Membrane, Cells, Cultured, Chemokine CCL2, Cytosol, Fibroblasts, Gene Expression, I-kappa B Proteins, Interleukin-1 Receptor Accessory Protein, Interleukin-1 Receptor-Associated Kinases, Interleukin-1beta, Interleukin-6, Leupeptins, Lipopolysaccharides, Mice, Mice, Knockout, Myeloid Differentiation Factor 88, NF-kappa B, Proteasome Endopeptidase Complex, Protein Binding, Receptors, Tumor Necrosis Factor, Type I, Signal Transduction, Tumor Necrosis Factor-alpha, Ubiquitination
Animals, Cell Membrane, Cells, Cultured, Chemokine CCL2, Cytosol, Fibroblasts, Gene Expression, I-kappa B Proteins, Interleukin-1 Receptor Accessory Protein, Interleukin-1 Receptor-Associated Kinases, Interleukin-1beta, Interleukin-6, Leupeptins, Lipopolysaccharides, Mice, Mice, Knockout, Myeloid Differentiation Factor 88, NF-kappa B, Proteasome Endopeptidase Complex, Protein Binding, Receptors, Tumor Necrosis Factor, Type I, Signal Transduction, Tumor Necrosis Factor-alpha, Ubiquitination
Am. J. Physiol., Cell Physiol.
Date: Aug. 01, 2008
PubMed ID: 18562480
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