Repression of transcriptional activity of estrogen receptor alpha by a Cullin3/SPOP ubiquitin E3 ligase complex.
The role of SPOP in the ubiquitination of ER alpha by the Cullin3-based E3 ubiquitin ligase complex was investigated. We showed that the N-terminal region of SPOP containing the MATH domain interacts with the AF-2 domain of ER alpha in cultured human embryonic 293 cells. SPOP was required for coimmunoprecipitation ... of ER alpha; with Cullin3. This is the first report of the essential role of SPOP in ERalpha ubiquitination by the Cullin3-based E3 ubiquitin ligase complex. We also demonstrated repression of the transactivation capability of ER alpha; in cultured mammalian cells.
Mesh Terms:
Cell Line, Cullin Proteins, Estrogen Receptor alpha, Humans, Protein Binding, Repressor Proteins, Transcription, Genetic, Transcriptional Activation, Ubiquitin-Protein Ligases, Ubiquitination
Cell Line, Cullin Proteins, Estrogen Receptor alpha, Humans, Protein Binding, Repressor Proteins, Transcription, Genetic, Transcriptional Activation, Ubiquitin-Protein Ligases, Ubiquitination
Mol. Cells
Date: Apr. 30, 2008
PubMed ID: 18414007
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- Interactions 3
- PTM Genes 1