A molecular determinant for the establishment of sister chromatid cohesion.
Chromosome segregation, transcriptional regulation, and repair of DNA double-strand breaks require the cohesin protein complex. Cohesin holds the replicated chromosomes (sister chromatids) together to mediate sister chromatid cohesion. The mechanism of how cohesion is established is unknown. We found that in budding yeast, the head domain of the Smc3p subunit ... of cohesin is acetylated by the Eco1p acetyltransferase at two evolutionarily conserved residues, promoting the chromatin-bound cohesin to tether sister chromatids. Smc3p acetylation is induced in S phase after the chromatin loading of cohesin and is suppressed in G(1) and G(2)/M. Smc3 head acetylation and its cell cycle regulation provide important insights into the biology and mechanism of cohesion establishment.
Mesh Terms:
Acetylation, Acetyltransferases, Amino Acid Sequence, Amino Acid Substitution, Cell Cycle Proteins, Cell Division, Chromatids, Chromatin, Chromosomal Proteins, Non-Histone, Chromosomes, Fungal, G1 Phase, G2 Phase, Immunoprecipitation, Lysine, Molecular Sequence Data, Mutation, Nuclear Proteins, Protein Structure, Tertiary, Proteochondroitin Sulfates, S Phase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Acetylation, Acetyltransferases, Amino Acid Sequence, Amino Acid Substitution, Cell Cycle Proteins, Cell Division, Chromatids, Chromatin, Chromosomal Proteins, Non-Histone, Chromosomes, Fungal, G1 Phase, G2 Phase, Immunoprecipitation, Lysine, Molecular Sequence Data, Mutation, Nuclear Proteins, Protein Structure, Tertiary, Proteochondroitin Sulfates, S Phase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Science
Date: Jul. 25, 2008
PubMed ID: 18653894
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