Identification and characterization of a family of Rab11-interacting proteins.
Rab11a is a small GTP-binding protein enriched in the pericentriolar plasma membrane recycling systems. We hypothesized that Rab11a-binding proteins exist as downstream effectors of its action. Here we define a family of four Rab11-interacting proteins: Rab11-Family Interacting Protein 1 (Rab11-FIP1), Rab11-Family Interacting Protein 2 (Rab11-FIP2), Rab11-Family Interacting Protein 3 (Rab11-FIP3), ... and pp75/Rip11. All four interacting proteins associated with wild type Rab11a and dominant active Rab11a (Rab11aS20V) as well as Rab11b and Rab25. Rab11-FIP2 also interacted with dominant negative Rab11a (Rab11aS25N) and the tail of myosin Vb. The binding of Rab11-FIP1, Rab11-FIP2, and Rab11-FIP3 to Rab11a was dependent upon a conserved carboxyl-terminal amphipathic alpha-helix. Rab11-FIP1, Rab11-FIP2, and pp75/Rip11 colocalized with Rab11a in plasma membrane recycling systems in both non-polarized HeLa cells and polarized Madin-Darby canine kidney cells. GFP-Rab11-FIP3 also colocalized with Rab11a in HeLa cells. Rab11-FIP1, Rab11-FIP2, and pp75/Rip11 also coenriched with Rab11a and H(+)K(+)-ATPase on parietal cell tubulovesicles, and Rab11-FIP1 and Rab11-FIP2 translocated with Rab11a and the H(+)K(+)-ATPase upon stimulating parietal cells with histamine. The results suggest that the function of Rab11a in plasma membrane recycling systems is dependent upon a compendium of protein effectors.
Mesh Terms:
Amino Acid Sequence, Animals, Antineoplastic Agents, Antineoplastic Agents, Phytogenic, Base Sequence, Blotting, Western, Cell Line, Cell Membrane, Cloning, Molecular, Conserved Sequence, DNA, Complementary, Dogs, Expressed Sequence Tags, Gastric Mucosa, Gene Deletion, Gene Library, Genes, Dominant, Hela Cells, Histamine, Humans, Microscopy, Fluorescence, Molecular Sequence Data, Nocodazole, Paclitaxel, Protein Binding, Rabbits, Recombinant Proteins, Sequence Homology, Amino Acid, Two-Hybrid System Techniques, rab GTP-Binding Proteins
Amino Acid Sequence, Animals, Antineoplastic Agents, Antineoplastic Agents, Phytogenic, Base Sequence, Blotting, Western, Cell Line, Cell Membrane, Cloning, Molecular, Conserved Sequence, DNA, Complementary, Dogs, Expressed Sequence Tags, Gastric Mucosa, Gene Deletion, Gene Library, Genes, Dominant, Hela Cells, Histamine, Humans, Microscopy, Fluorescence, Molecular Sequence Data, Nocodazole, Paclitaxel, Protein Binding, Rabbits, Recombinant Proteins, Sequence Homology, Amino Acid, Two-Hybrid System Techniques, rab GTP-Binding Proteins
J. Biol. Chem.
Date: Oct. 19, 2001
PubMed ID: 11495908
View in: Pubmed Google Scholar
Download Curated Data For This Publication
8633
Switch View:
- Interactions 15