Histone H2A monoubiquitination represses transcription by inhibiting RNA polymerase II transcriptional elongation.
Solving the biological roles of covalent histone modifications, including monoubiquitination of histone H2A, and the molecular mechanisms by which these modifications regulate specific transcriptional programs remains a central question for all eukaryotes. Here we report that the N-CoR/HDAC1/3 complex specifically recruits a specific histone H2A ubiquitin ligase, 2A-HUB/hRUL138, to a ... subset of regulated gene promoters. 2A-HUB catalyzes monoubiquitination of H2A at lysine 119, functioning as a combinatoric component of the repression machinery required for specific gene regulation programs. Thus, 2A-HUB mediates a selective repression of a specific set of chemokine genes in macrophages, critically modulating migratory responses to TLR activation. H2A monoubiquitination acts to prevent FACT recruitment at the transcriptional promoter region, blocking RNA polymerase II release at the early stage of elongation. We suggest that distinct H2A ubiquitinases, each recruited based on interactions with different corepressor complexes, contribute to distinct transcriptional repression programs.
Mesh Terms:
Amino Acid Sequence, Animals, Cell Line, Chemokines, DNA-Binding Proteins, High Mobility Group Proteins, Histone Deacetylase 1, Histone Deacetylases, Histones, Humans, Ligases, Macrophages, Mice, Molecular Sequence Data, Nuclear Proteins, Nuclear Receptor Co-Repressor 1, Nuclear Receptor Co-Repressor 2, Peptide Chain Elongation, Translational, Protein Processing, Post-Translational, RING Finger Domains, RNA Polymerase II, RNA-Binding Proteins, Repressor Proteins, Transcription, Genetic, Transcriptional Elongation Factors, Ubiquitin, Ubiquitination
Amino Acid Sequence, Animals, Cell Line, Chemokines, DNA-Binding Proteins, High Mobility Group Proteins, Histone Deacetylase 1, Histone Deacetylases, Histones, Humans, Ligases, Macrophages, Mice, Molecular Sequence Data, Nuclear Proteins, Nuclear Receptor Co-Repressor 1, Nuclear Receptor Co-Repressor 2, Peptide Chain Elongation, Translational, Protein Processing, Post-Translational, RING Finger Domains, RNA Polymerase II, RNA-Binding Proteins, Repressor Proteins, Transcription, Genetic, Transcriptional Elongation Factors, Ubiquitin, Ubiquitination
Mol. Cell
Date: Jan. 18, 2008
PubMed ID: 18206970
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