WRKY group IId transcription factors interact with calmodulin.

Calmodulin (CaM) is a ubiquitous Ca(2+)-binding protein known to regulate diverse cellular functions by modulating the activity of various target proteins. We isolated a cDNA encoding AtWRKY7, a novel CaM-binding transcription factor, from an Arabidopsis expression library with horseradish peroxidase-conjugated CaM. CaM binds specifically to the Ca(2+)-dependent CaM-binding domain (CaMBD) ...
of AtWRKY7, as shown by site-directed mutagenesis, a gel mobility shift assay, a split-ubiquitin assay, and a competition assay using a Ca2+/CaM-dependent enzyme. Furthermore, we show that the CaMBD of AtWRKY7 is a conserved structural motif (C-motif) found in group IId of the WRKY protein family.
Mesh Terms:
Amino Acid Sequence, Arabidopsis, Arabidopsis Proteins, Calmodulin, Calmodulin-Binding Proteins, Molecular Sequence Data, Peptide Fragments, Phylogeny, Protein Binding, Sequence Alignment, Transcription Factors, Ubiquitin
FEBS Lett.
Date: Feb. 28, 2005
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