Identification of two proteins, S14 and UIP1, that interact with UCH37.
By the use of the yeast two-hybrid screen we have identified two proteins that interacted with UCH37: S14, which is a subunit of PA700 and a novel protein, UIP1 (UCH37 interacting protein 1). The interaction of UCH37 with S14 or UIP1 was confirmed by in vitro binding assay and in ... vivo co-immunoprecipitation analysis. The C-terminal extension of UCH37 is essential for interaction with S14 or UIP1 as shown by the yeast two-hybrid assay and the in vitro binding assay. Furthermore, UIP1 blocked the interaction between UCH37 and S14 in vitro.
Mesh Terms:
Amino Acid Sequence, Binding Sites, Binding, Competitive, Blotting, Western, Carboxypeptidases, Carrier Proteins, Gene Expression Profiling, Humans, Molecular Sequence Data, Precipitin Tests, Proteasome Endopeptidase Complex, Protein Binding, Protein Subunits, Proteins, RNA, Messenger, Sequence Alignment, Thiolester Hydrolases, Two-Hybrid System Techniques, Ubiquitin Thiolesterase
Amino Acid Sequence, Binding Sites, Binding, Competitive, Blotting, Western, Carboxypeptidases, Carrier Proteins, Gene Expression Profiling, Humans, Molecular Sequence Data, Precipitin Tests, Proteasome Endopeptidase Complex, Protein Binding, Protein Subunits, Proteins, RNA, Messenger, Sequence Alignment, Thiolester Hydrolases, Two-Hybrid System Techniques, Ubiquitin Thiolesterase
FEBS Lett.
Date: Jan. 19, 2001
PubMed ID: 11163772
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