Cyclin D1 and p22ack1 play opposite roles in plant growth and development.
The plant cell division cycle, a highly coordinated process, is continually regulated during the growth and development of plants. In this report, we demonstrate how two cell-cycle regulators act together to control cell proliferation in transgenic Arabidopsis. To identify potential cyclin dependent kinase regulators from Arabidopsis, we employed an two-hybrid ... screening system to isolate genes encoding G1 specific cyclin-interacting proteins. One of these, p22(ack1), which encodes a novel 22kDa protein, binds to cyclin D1. Overexpression of p22(ack1) in transgenic Arabidopsis resulted in growth retardation due to a strong inhibition of cell division in the leaf primordial and meristematic tissue. The leaf shape of p22(ack1) transgenic Arabidopsis was altered from oval in wild-type to dentate. Wild-type phenotype was successfully restored in F1 hybrids by cross-hybridizing the p22(ackl)Arabidopsis mutants with cyclin D1. Taken together, these results suggest that p22(ack1) and cyclin D1, which act antagonistically, are major rate-limiting factors for cell division in the leaf meristem.
Mesh Terms:
Arabidopsis, Arabidopsis Proteins, Carrier Proteins, Cell Cycle, Cell Cycle Proteins, Cyclin D1, In Situ Hybridization, Meristem, Phenotype, Plant Leaves, Plants, Genetically Modified, Two-Hybrid System Techniques
Arabidopsis, Arabidopsis Proteins, Carrier Proteins, Cell Cycle, Cell Cycle Proteins, Cyclin D1, In Situ Hybridization, Meristem, Phenotype, Plant Leaves, Plants, Genetically Modified, Two-Hybrid System Techniques
Biochem. Biophys. Res. Commun.
Date: Nov. 05, 2004
PubMed ID: 15464981
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