Collybistin, a newly identified brain-specific GEF, induces submembrane clustering of gephyrin.
The formation of postsynaptic GABAA and glycine receptor clusters requires the receptor-associated peripheral membrane protein gephyrin. Here we describe two splice variants of a novel gephyrin-binding protein, termed collybistin I and II, which belong to the family of dbl-like GDP/GTP exchange factors (GEFs). Co-expression of collybistin II with gephyrin induced ... the formation of submembrane gephyrin aggregates that accumulate hetero-oligomeric glycine receptors. Our data suggest that collybistin II regulates the membrane deposition of gephyrin by activating a GTPase of the Rho/Rac family. Therefore, this protein may be an important determinant of inhibitory postsynaptic membrane formation and plasticity.
Mesh Terms:
Alternative Splicing, Amino Acid Sequence, Animals, Base Sequence, Brain, Carrier Proteins, Cell Line, Conserved Sequence, GTP Phosphohydrolases, Guanine Nucleotide Exchange Factors, Humans, Macromolecular Substances, Membrane Proteins, Molecular Sequence Data, Organ Specificity, Protein Isoforms, Rats, Receptors, Glycine, Sequence Homology, Amino Acid, Synapses, Synaptic Membranes, src Homology Domains
Alternative Splicing, Amino Acid Sequence, Animals, Base Sequence, Brain, Carrier Proteins, Cell Line, Conserved Sequence, GTP Phosphohydrolases, Guanine Nucleotide Exchange Factors, Humans, Macromolecular Substances, Membrane Proteins, Molecular Sequence Data, Organ Specificity, Protein Isoforms, Rats, Receptors, Glycine, Sequence Homology, Amino Acid, Synapses, Synaptic Membranes, src Homology Domains
Nat. Neurosci.
Date: Jan. 01, 2000
PubMed ID: 10607391
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