hFOG-2, a novel zinc finger protein, binds the co-repressor mCtBP2 and modulates GATA-mediated activation.

We have identified a novel human zinc finger protein, hFOG-2, which is related to but distinct from the murine transcription factor Friend-of-GATA-1 (mFOG-1). The hFOG-2 gene was initially detected in K562 cells using a polymerase chain reaction approach with degenerate primers corresponding to zinc finger regions of mFOG-1. A murine ...
homologue of hFOG-2 was also identified in the mouse expressed sequence tag data banks, indicating that a family of FOG genes exists in mammals. hFOG-2 appears to be widely expressed, while mFOG-1 is expressed primarily in erythroid and megakaryocytic cells and plays a fundamental role in the development of these lineages. Sequencing of the full-length hFOG-2 cDNA indicates that the interaction domains for transcription factors GATA-1 and mCtBP2 are both conserved and we have shown that this new FOG protein also physically interacts with these factors. We have demonstrated that hFOG-2, like mFOG-1, can act in concert with GATA-1 to activate gene expression from the p45 NF-E2 promoter region, but that it can also act to repress GATA-mediated activation of additional reporter constructs. Finally, we have identified a repression domain in hFOG-2 and show that repression is dependent upon the integrity of the mCtBP2 interaction motif Pro-Ile-Asp-Leu-Ser.
Mesh Terms:
Amino Acid Sequence, Animals, Base Sequence, Cell Line, Cloning, Molecular, DNA Primers, DNA-Binding Proteins, Erythroid-Specific DNA-Binding Factors, GATA1 Transcription Factor, Humans, Mice, Molecular Sequence Data, NF-E2 Transcription Factor, NF-E2 Transcription Factor, p45 Subunit, Phosphoproteins, Promoter Regions, Genetic, Protein Binding, Repressor Proteins, Sequence Homology, Amino Acid, Transcription Factors, Zinc Fingers
J. Biol. Chem.
Date: Aug. 13, 1999
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