hFOG-2, a novel zinc finger protein, binds the co-repressor mCtBP2 and modulates GATA-mediated activation.
We have identified a novel human zinc finger protein, hFOG-2, which is related to but distinct from the murine transcription factor Friend-of-GATA-1 (mFOG-1). The hFOG-2 gene was initially detected in K562 cells using a polymerase chain reaction approach with degenerate primers corresponding to zinc finger regions of mFOG-1. A murine ... homologue of hFOG-2 was also identified in the mouse expressed sequence tag data banks, indicating that a family of FOG genes exists in mammals. hFOG-2 appears to be widely expressed, while mFOG-1 is expressed primarily in erythroid and megakaryocytic cells and plays a fundamental role in the development of these lineages. Sequencing of the full-length hFOG-2 cDNA indicates that the interaction domains for transcription factors GATA-1 and mCtBP2 are both conserved and we have shown that this new FOG protein also physically interacts with these factors. We have demonstrated that hFOG-2, like mFOG-1, can act in concert with GATA-1 to activate gene expression from the p45 NF-E2 promoter region, but that it can also act to repress GATA-mediated activation of additional reporter constructs. Finally, we have identified a repression domain in hFOG-2 and show that repression is dependent upon the integrity of the mCtBP2 interaction motif Pro-Ile-Asp-Leu-Ser.
Mesh Terms:
Amino Acid Sequence, Animals, Base Sequence, Cell Line, Cloning, Molecular, DNA Primers, DNA-Binding Proteins, Erythroid-Specific DNA-Binding Factors, GATA1 Transcription Factor, Humans, Mice, Molecular Sequence Data, NF-E2 Transcription Factor, NF-E2 Transcription Factor, p45 Subunit, Phosphoproteins, Promoter Regions, Genetic, Protein Binding, Repressor Proteins, Sequence Homology, Amino Acid, Transcription Factors, Zinc Fingers
Amino Acid Sequence, Animals, Base Sequence, Cell Line, Cloning, Molecular, DNA Primers, DNA-Binding Proteins, Erythroid-Specific DNA-Binding Factors, GATA1 Transcription Factor, Humans, Mice, Molecular Sequence Data, NF-E2 Transcription Factor, NF-E2 Transcription Factor, p45 Subunit, Phosphoproteins, Promoter Regions, Genetic, Protein Binding, Repressor Proteins, Sequence Homology, Amino Acid, Transcription Factors, Zinc Fingers
J. Biol. Chem.
Date: Aug. 13, 1999
PubMed ID: 10438528
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