[Screening of proteins interact with FMR1 by yeast two-hybrid system]
OBJECTIVE: To establish yeast two-hybrid system for screening of protein(s) interacted with the fragile X metal retardation protein (FMRP). METHODS: Fragment of exon 11 to 15 of the FMR1 cDNA was recombined with DNA-binding domain of the pBTM116 vector as bait to screen a mouse embryo cDNA library. RESULTS: Thirteen ... clones were confirmed to be able to specifically interact with FMRP bait. Sequence analysis showed that 12 clones are overlapping ones containing cDNA fragments of the mouse ubiquitin-conjugating enzyme gene (mUBC9). CONCLUSIONS: The interaction between UBC9 and FMRP is supported both by similarity search of the amino acid sequences of the mouse and human UBC9 and by expression characteristics of the two proteins. The biological significance of the interaction is to be further studied.
Mesh Terms:
Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA-Binding Proteins, Embryo, Mammalian, Exons, Fragile X Mental Retardation Protein, Gene Library, Ligases, Mice, Molecular Sequence Data, Nerve Tissue Proteins, RNA-Binding Proteins, Sequence Analysis, Protein, Ubiquitin-Conjugating Enzymes
Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA-Binding Proteins, Embryo, Mammalian, Exons, Fragile X Mental Retardation Protein, Gene Library, Ligases, Mice, Molecular Sequence Data, Nerve Tissue Proteins, RNA-Binding Proteins, Sequence Analysis, Protein, Ubiquitin-Conjugating Enzymes
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
Date: Jun. 01, 1998
PubMed ID: 11367701
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