The Cdk-like protein PCTAIRE-1 from mouse brain associates with p11 and 14-3-3 proteins.
PCTAIRE-1 is a member of the cyclin-dependent kinase (cdk)-like class of proteins, and is localized mainly in the mammalian brain. Using the yeast two-hybrid system we screened a mouse brain cDNA library with PCTAIRE-1 as bait, and isolated several clones coding for the mouse homologs of the following proteins: p11 ... (also known as calpactin I light chain) and the eta, theta (also known as tau) and zeta isoforms of 14-3-3 proteins. We confirmed that these four proteins interact with PCTAIRE-1 by demonstrating the biochemical interactions using the pure recombinant proteins. The fact that 14-3-3 proteins are known to interact with many other intracellular proteins (such as C-kinase, Raf, Bcr, P13-kinase) and p11 with annexin II (a major pp60(v-src) and C-kinase substrate) suggests that PCTAIRE-1 might be part of multiple signal transduction cascades and cellular protein networks.
Mesh Terms:
14-3-3 Proteins, Amino Acid Sequence, Animals, Annexin A2, Base Sequence, Brain, Cloning, Molecular, Cyclin-Dependent Kinases, Mice, Molecular Sequence Data, Molecular Weight, Protein Binding, Protein-Serine-Threonine Kinases, Proteins, RNA, Messenger, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Tyrosine 3-Monooxygenase
14-3-3 Proteins, Amino Acid Sequence, Animals, Annexin A2, Base Sequence, Brain, Cloning, Molecular, Cyclin-Dependent Kinases, Mice, Molecular Sequence Data, Molecular Weight, Protein Binding, Protein-Serine-Threonine Kinases, Proteins, RNA, Messenger, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Tyrosine 3-Monooxygenase
Mol. Gen. Genet.
Date: May. 20, 1997
PubMed ID: 9197417
View in: Pubmed Google Scholar
Download Curated Data For This Publication
8828
Switch View:
- Interactions 4