Bax inhibitor-1, a mammalian apoptosis suppressor identified by functional screening in yeast.

The mammalian proapoptotic protein Bax confers a lethal phenotype when expressed in yeast. By exploiting this phenotype, we have identified a novel human Bax inhibitor, BI-1. BI-1 is an evolutionarily conserved integral membrane protein containing multiple membrane-spanning segments and is predominantly localized to intracellular membranes, similar to Bcl-2 family proteins. ...
Moreover, BI-1 can interact with Bcl-2 and Bcl-XL but Bax or Bak, as demonstrated by in vivo cross-linking and coimmunoprecipitation studies. When overexpressed in mammalian cells, BI-1 suppressed apoptosis included by Bax, etoposide, staurosporine, and growth factor deprivation, but not by Fas (CD95). Conversely, BI-1 antisense induced apoptosis. BI-1 thus represents a new type of regulator of cell death pathways controlled by Bcl-2 and Bax.
Mesh Terms:
Animals, Antigens, CD95, Antineoplastic Agents, Phytogenic, Apoptosis, Apoptosis Regulatory Proteins, COS Cells, Carcinogens, DNA, Antisense, DNA, Complementary, Etoposide, Fibroblasts, Gene Expression Regulation, Fungal, Gene Library, Genetic Testing, Growth Substances, Humans, Intracellular Membranes, Mammals, Membrane Proteins, Molecular Sequence Data, Phenotype, Proteins, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-bcl-2, Sequence Homology, Amino Acid, Staurosporine, Transfection, Yeasts, bcl-2-Associated X Protein
Mol. Cell
Date: Feb. 01, 1998
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