Physical association of beta 2 integrin with GPI-80, a novel glycosylphosphatidylinositol-anchored protein with potential for regulating adhesion and migration.
We recently found a novel GPI-anchored protein, GPI-80, on human phagocytes that may regulate leukocyte adherence, locomotion, and extravasation. To clarify the mechanisms by which GPI-80 functions on leukocytes, we explored the possibility of its physical association with beta 2 integrin which is important for leukocyte adherence, locomotion, and extravasation. ... beta 2 integrin, detected by anti-CD18 mAb, was coprecipitated with GPI-80 from human neutrophil lysates by a mAb to GPI-80. In addition, GPI-80 was immunoprecipitated from human neutrophil lysates by anti-human CD18 mAb. These results clearly show that GPI-80 is physically associated with beta 2 integrin in human neutrophils.
Mesh Terms:
Antigens, CD18, Cell Adhesion, Cell Adhesion Molecules, Cell Movement, Humans, Hydrolases, Neutrophils, Precipitin Tests, Protein Binding
Antigens, CD18, Cell Adhesion, Cell Adhesion Molecules, Cell Movement, Humans, Hydrolases, Neutrophils, Precipitin Tests, Protein Binding
Biochem. Biophys. Res. Commun.
Date: Jun. 14, 2002
PubMed ID: 12056825
View in: Pubmed Google Scholar
Download Curated Data For This Publication
9007
Switch View:
- Interactions 1