Dendrite development regulated by CREST, a calcium-regulated transcriptional activator.

The lasting effects of neuronal activity on brain development involve calcium-dependent gene expression. Using a strategy called transactivator trap, we cloned a calcium-responsive transactivator called CREST (for calcium-responsive transactivator). CREST is a SYT-related nuclear protein that interacts with adenosine 3',5'-monophosphate (cAMP) response element-binding protein (CREB)-binding protein (CBP) and is expressed ...
in the developing brain. Mice that have a targeted disruption of the crest gene are viable but display defects in cortical and hippocampal dendrite development. Cortical neurons from crest mutant mice are compromised in calcium-dependent dendritic growth. Thus, calcium activation of CREST-mediated transcription helps regulate neuronal morphogenesis.
Mesh Terms:
Amino Acid Sequence, Animals, Blotting, Northern, Brain, CREB-Binding Protein, Calcium, Calcium Channels, Cell Line, Cells, Cultured, Cerebral Cortex, Cloning, Molecular, Dendrites, Gene Expression Profiling, Gene Expression Regulation, Developmental, Gene Library, Gene Targeting, Humans, In Situ Hybridization, Mice, Mice, Knockout, Molecular Sequence Data, Mutation, Nervous System, Neurons, Nuclear Proteins, Protein Structure, Tertiary, Rats, Recombinant Fusion Proteins, Trans-Activators, Transcription, Genetic, Transcriptional Activation, Transfection
Science
Date: Jan. 09, 2004
Download Curated Data For This Publication
9128
Switch View:
  • Interactions 3