The beta2-adaptin clathrin adaptor interacts with the mitotic checkpoint kinase BubR1.
The adaptor AP2 is a heterotetrameric complex that associates with clathrin and regulatory proteins to mediate rapid endocytosis from the plasma membrane. Here, we report the identification of the mitotic checkpoint kinase BubR1 as a novel binding partner of beta2-adaptin, one of the AP2 large subunits. Using two-hybrid experiments and ... in vitro binding assays, we show that beta2-adaptin binds to BubR1 through its amino-terminal beta2-'trunk' domain, while the beta2-binding region of BubR1 maps to the carboxy-terminal kinase domain. Subcellular immunolocalization studies suggest that the interaction between BubR1 and beta2-adaptin could take place in the cytosol at any time during the cell cycle. In addition, we found that BubR1 and the BubR1-related kinase, Bub1, also bind to beta-adaptins of other AP complexes. Together, these results support a model in which the mitotic checkpoint kinases BubR1 and BuB1, by binding to beta-adaptins, may play novel roles in the regulation of vesicular intracellular traffic.
Mesh Terms:
Adaptor Protein Complex beta Subunits, Base Sequence, Binding Sites, Cell Cycle Proteins, DNA, Complementary, Humans, Immunohistochemistry, Mitosis, Models, Biological, Peptide Mapping, Protein Binding, Protein Kinases, Protein Structure, Tertiary, Protein Subunits, Protein-Serine-Threonine Kinases, Recombinant Proteins, Two-Hybrid System Techniques
Adaptor Protein Complex beta Subunits, Base Sequence, Binding Sites, Cell Cycle Proteins, DNA, Complementary, Humans, Immunohistochemistry, Mitosis, Models, Biological, Peptide Mapping, Protein Binding, Protein Kinases, Protein Structure, Tertiary, Protein Subunits, Protein-Serine-Threonine Kinases, Recombinant Proteins, Two-Hybrid System Techniques
Biochem. Biophys. Res. Commun.
Date: Nov. 15, 2002
PubMed ID: 12419313
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