PRDI-BF1 recruits the histone H3 methyltransferase G9a in transcriptional silencing.
PRDI-BF1, the human ortholog of mouse Blimp-1, is a DNA-binding protein involved in postinduction repression of interferon-beta gene transcription in response to viral infection. PRDI-BF1 also has an essential function in driving terminal differentiation of B lymphocytes and therein silences multiple genes. Here we show PRDI-BF1 assembles silent chromatin over ... the interferon-beta promoter in the osteosarcoma cell line U2OS through recruitment of the histone H3 lysine methyltransferase G9a. G9a is recruited only when in a complex with PRDI-BF1. G9a catalytic activity is required for the accumulation of methylated histone H3 and transcriptional silencing mediated by PRDI-BF1 in vivo. This establishes a mechanism for the recruitment of G9a, the main mammalian euchromatic methyltransferase, and defines nonembryonic targets of G9a.
Mesh Terms:
Amino Acid Sequence, Cell Line, Tumor, Chromatin, Gene Silencing, Histone-Lysine N-Methyltransferase, Humans, Interferon-beta, Macromolecular Substances, Methyltransferases, Molecular Sequence Data, Protein Methyltransferases, Protein Transport, Repressor Proteins, Substrate Specificity, Transcription Factors, Transcription, Genetic
Amino Acid Sequence, Cell Line, Tumor, Chromatin, Gene Silencing, Histone-Lysine N-Methyltransferase, Humans, Interferon-beta, Macromolecular Substances, Methyltransferases, Molecular Sequence Data, Protein Methyltransferases, Protein Transport, Repressor Proteins, Substrate Specificity, Transcription Factors, Transcription, Genetic
Nat. Immunol.
Date: Mar. 01, 2004
PubMed ID: 14985713
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