Sgt1 dimerization is negatively regulated by protein kinase CK2-mediated phosphorylation at Ser361.
The kinetochore, which consists of centromere DNA and structural proteins, is essential for proper chromosome segregation in eukaryotes. In budding yeast, Sgt1 and Hsp90 are required for the binding of Skp1 to Ctf13 (a component of the core kinetochore complex CBF3) and therefore for the assembly of CBF3. We have ... previously shown that Sgt1 dimerization is important for this kinetochore assembly mechanism. In this study, we report that protein kinase CK2 phosphorylates Ser(361) on Sgt1, and this phosphorylation inhibits Sgt1 dimerization.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Casein Kinase II, HSP90 Heat-Shock Proteins, Humans, Kinetochores, Molecular Sequence Data, Nuclear Proteins, Phosphorylation, Plasmids, Protein Binding, Protein Multimerization, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Serine, Time Factors
Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Casein Kinase II, HSP90 Heat-Shock Proteins, Humans, Kinetochores, Molecular Sequence Data, Nuclear Proteins, Phosphorylation, Plasmids, Protein Binding, Protein Multimerization, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Serine, Time Factors
J. Biol. Chem.
Date: Jul. 10, 2009
PubMed ID: 19398558
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