Identification, structure, and functional requirement of the Mediator submodule Med7N/31.

Mediator is a modular multiprotein complex required for regulated transcription by RNA polymerase (Pol) II. Here, we show that the middle module of the Mediator core contains a submodule of unique structure and function that comprises the N-terminal part of subunit Med7 (Med7N) and the highly conserved subunit Med31 (Soh1). ...
The Med7N/31 submodule shows a conserved novel fold, with two proline-rich stretches in Med7N wrapping around the right-handed four-helix bundle of Med31. In vitro, Med7N/31 is required for activated transcription and can act in trans when added exogenously. In vivo, Med7N/31 has a predominantly positive function on the expression of a specific subset of genes, including genes involved in methionine metabolism and iron transport. Comparative phenotyping and transcriptome profiling identify specific and overlapping functions of different Mediator submodules.
Mesh Terms:
Amino Acid Sequence, Crystallography, X-Ray, Drosophila Proteins, Genetic Complementation Test, Mediator Complex, Models, Molecular, Molecular Sequence Data, Protein Structure, Quaternary, Saccharomyces cerevisiae, Sequence Alignment, Trans-Activators, Transcription Factors, Transcription, Genetic
EMBO J.
Date: Jan. 07, 2009
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