Brahma links the SWI/SNF chromatin-remodeling complex with MeCP2-dependent transcriptional silencing.
Transcriptional repression of methylated genes can be mediated by the methyl-CpG binding protein MeCP2. Here we show that human Brahma (Brm), a catalytic component of the SWI/SNF-related chromatin-remodeling complex, associates with MeCP2 in vivo and is functionally linked with repression. We used a number of different molecular approaches and chromatin ... immunoprecipitation strategies to show a unique cooperation between Brm, BAF57 and MeCP2. We show that Brm and MeCP2 assembly on chromatin occurs on methylated genes in cancer and the gene FMR1 in fragile X syndrome. These experimental findings identify a new role for SWI/SNF in gene repression by MeCP2.
Mesh Terms:
Animals, Cell Cycle Proteins, Chromosomal Proteins, Non-Histone, DNA-Binding Proteins, Drosophila Proteins, Fragile X Mental Retardation Protein, Gene Silencing, Histones, Humans, Methyl-CpG-Binding Protein 2, Mice, Microscopy, Fluorescence, Molecular Sequence Data, NIH 3T3 Cells, Nerve Tissue Proteins, RNA-Binding Proteins, Repressor Proteins, Trans-Activators, Transcription Factors
Animals, Cell Cycle Proteins, Chromosomal Proteins, Non-Histone, DNA-Binding Proteins, Drosophila Proteins, Fragile X Mental Retardation Protein, Gene Silencing, Histones, Humans, Methyl-CpG-Binding Protein 2, Mice, Microscopy, Fluorescence, Molecular Sequence Data, NIH 3T3 Cells, Nerve Tissue Proteins, RNA-Binding Proteins, Repressor Proteins, Trans-Activators, Transcription Factors
Nat. Genet.
Date: Mar. 01, 2005
PubMed ID: 15696166
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