Transcription repression activity is associated with the type I isoform of the MMSET gene involved in t(4;14) in multiple myeloma.
The WHSC1/MMSET gene, involved in t(4;14)(p16.3;q32) in multiple myeloma, encodes putative isoforms (MMSET I, MMSET II and RE-IIBP) which are thought to be involved in transcription regulation. We investigated their activity in transfected 293T and HeLa cells. Both MMSET I and MMSET II were localised in the nucleus, whereas RE-IIBP ... showed cytoplasmic and nucleolar staining. MMSET I dose-dependently repressed the transcriptional activity of the promoter region of the thymidine kinase gene, whereas MMSET II and RE-IIBP had no effect. The HDAC inhibitor, trichostatin A, reduced MMSET I repression activity and in vitro co-immunoprecipitation analyses indicated that MMSET I specifically recruits HDAC1 and mSin3b, but not HDAC2 or HDAC4. Our data support the hypothesis that MMSET may act as a transcription regulator; different functions may be associated with distinct isoforms.
Mesh Terms:
Carrier Proteins, Cell Line, Chromosomes, Human, Pair 14, Chromosomes, Human, Pair 4, Gene Deletion, Gene Expression Regulation, Neoplastic, Genes, Regulator, Hela Cells, Histone-Lysine N-Methyltransferase, Humans, Multiple Myeloma, Protein Isoforms, Protein Structure, Tertiary, Repressor Proteins, Transcription, Genetic
Carrier Proteins, Cell Line, Chromosomes, Human, Pair 14, Chromosomes, Human, Pair 4, Gene Deletion, Gene Expression Regulation, Neoplastic, Genes, Regulator, Hela Cells, Histone-Lysine N-Methyltransferase, Humans, Multiple Myeloma, Protein Isoforms, Protein Structure, Tertiary, Repressor Proteins, Transcription, Genetic
Br. J. Haematol.
Date: Oct. 01, 2005
PubMed ID: 16197452
View in: Pubmed Google Scholar
Download Curated Data For This Publication
93118
Switch View:
- Interactions 4