ESCRT ubiquitin-binding domains function cooperatively during MVB cargo sorting.
Ubiquitin (Ub) sorting receptors facilitate the targeting of ubiquitinated membrane proteins into multivesicular bodies (MVBs). Ub-binding domains (UBDs) have been described in several endosomal sorting complexes required for transport (ESCRT). Using available structural information, we have investigated the role of the multiple UBDs within ESCRTs during MVB cargo selection. We ... found a novel UBD within ESCRT-I and show that it contributes to MVB sorting in concert with the known UBDs within the ESCRT complexes. These experiments reveal an unexpected level of coordination among the ESCRT UBDs, suggesting that they collectively recognize a diverse set of cargo rather than act sequentially at discrete steps.
Mesh Terms:
Amino Acid Sequence, Animals, Carboxypeptidases, Endosomal Sorting Complexes Required for Transport, Endosomes, Membrane Proteins, Models, Molecular, Molecular Sequence Data, Multiprotein Complexes, Protein Binding, Protein Structure, Tertiary, Protein Transport, Receptors, Mating Factor, Recombinant Fusion Proteins, Saccharomyces cerevisiae Proteins, Ubiquitin, Vesicular Transport Proteins
Amino Acid Sequence, Animals, Carboxypeptidases, Endosomal Sorting Complexes Required for Transport, Endosomes, Membrane Proteins, Models, Molecular, Molecular Sequence Data, Multiprotein Complexes, Protein Binding, Protein Structure, Tertiary, Protein Transport, Receptors, Mating Factor, Recombinant Fusion Proteins, Saccharomyces cerevisiae Proteins, Ubiquitin, Vesicular Transport Proteins
J. Cell Biol.
Date: Apr. 20, 2009
PubMed ID: 19380877
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