JAZ repressor proteins are targets of the SCF(COI1) complex during jasmonate signalling.
Jasmonate and related signalling compounds have a crucial role in both host immunity and development in plants, but the molecular details of the signalling mechanism are poorly understood. Here we identify members of the jasmonate ZIM-domain (JAZ) protein family as key regulators of jasmonate signalling. JAZ1 protein acts to repress ... transcription of jasmonate-responsive genes. Jasmonate treatment causes JAZ1 degradation and this degradation is dependent on activities of the SCF(COI1) ubiquitin ligase and the 26S proteasome. Furthermore, the jasmonoyl-isoleucine (JA-Ile) conjugate, but not other jasmonate-derivatives such as jasmonate, 12-oxo-phytodienoic acid, or methyl-jasmonate, promotes physical interaction between COI1 and JAZ1 proteins in the absence of other plant proteins. Our results suggest a model in which jasmonate ligands promote the binding of the SCF(COI1) ubiquitin ligase to and subsequent degradation of the JAZ1 repressor protein, and implicate the SCF(COI1)-JAZ1 protein complex as a site of perception of the plant hormone JA-Ile.
Mesh Terms:
Amino Acid Sequence, Arabidopsis, Arabidopsis Proteins, Cell-Free System, Cyclopentanes, Genes, Plant, Glucuronidase, Isoleucine, Molecular Sequence Data, Multigene Family, Oxylipins, Phenotype, Protein Binding, Protein Structure, Tertiary, Repressor Proteins, SKP Cullin F-Box Protein Ligases, Signal Transduction, Substrate Specificity
Amino Acid Sequence, Arabidopsis, Arabidopsis Proteins, Cell-Free System, Cyclopentanes, Genes, Plant, Glucuronidase, Isoleucine, Molecular Sequence Data, Multigene Family, Oxylipins, Phenotype, Protein Binding, Protein Structure, Tertiary, Repressor Proteins, SKP Cullin F-Box Protein Ligases, Signal Transduction, Substrate Specificity
Nature
Date: Aug. 09, 2007
PubMed ID: 17637677
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