New insights into BS69 functions.
The BS69 protein has been commonly described as a co-repressor associated with various transcription factors. However, this hypothesis relied predominantly on overexpression of tagged proteins due to the lack of a reliable BS69 antibody. We present for the first time a complete sequence of BS69 and valuable tools to characterize ... the endogenous protein. We show that the full-length BS69 protein, as well as minor alternatively spliced isoforms, is ubiquitously expressed, nuclear, and associates with chromatin and mitotic chromosomes. Accordingly, BS69 interacts with a set of chromatin remodeling factors, including ATP-dependent helicases, histone deacetylases, and histone methyltransferases, as well as the E2F6 transcription factor. These data strengthen a role for BS69 in gene repression and link BS69 to chromatin remodeling.
Mesh Terms:
Alternative Splicing, Amino Acid Sequence, Animals, Carrier Proteins, Cell Line, Cell Nucleus, Chromatin, Histone-Lysine N-Methyltransferase, Humans, Mice, Molecular Sequence Data, Protein Isoforms, Protein Methyltransferases, Ubiquitin
Alternative Splicing, Amino Acid Sequence, Animals, Carrier Proteins, Cell Line, Cell Nucleus, Chromatin, Histone-Lysine N-Methyltransferase, Humans, Mice, Molecular Sequence Data, Protein Isoforms, Protein Methyltransferases, Ubiquitin
J. Biol. Chem.
Date: Jun. 16, 2006
PubMed ID: 16565076
View in: Pubmed Google Scholar
Download Curated Data For This Publication
93638
Switch View:
- Interactions 9